Biotechnology and Applied Biochemistry 1987-08-01

Thermally modified azocasein--a new insoluble substrate for the determination of proteolytic activity.

I Safarík

Index: Biotechnol. Appl. Biochem. 9 , 323-324, (1987)

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Abstract

An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot-air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate.