Indian Journal of Biochemistry and Biophysics 1990-10-01

Chromotropic character of bacterial acidic polysaccharides: Part III--Interaction of cationic dye pinacyanol chloride with Klebsiella K15 capsular polysaccharide.

A Mitra, A Chakrabarti, R K Nath, A K Chakraborty

Index: Indian J. Biochem. Biophys. 27(5) , 291-4, (1990)

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Abstract

Interaction of cationic dye pinacyanol chloride with the acidic capsular polysaccharide isolated from Klebsiella serotype K15 has been investigated by spectral measurements. Klebsiella K15 polysaccharide consists of hexasaccharide repeating units containing one residue each of glucuronic acid and glucose, and four residues of galactose. Glucuronic acid acts as the potential anionic site and the biopolymer interacts with the dye cations. It induces metachromasy in the dye and a blue shift of about 100 nm is observed in the visible absorption spectrum of the dye. Spectral measurements have been carried out at different polymer/dye molar ratios. Stoichiometry of polymer and dye in the polyanion-dye compound (1:1) indicates that every potential anionic site of the polyanion is associated with the dye cation, and stacking conformation is thus suggested. Effect of different non-aqueous solvents in reversing metachromasy has also been studied. Interaction studies exhibit chromotropic character of the biopolymer.