Journal of Molecular Biology 2015-08-14

Robust Antibody-Antigen Complexes Prediction Generated by Combining Sequence Analyses, Mutagenesis, In Vitro Evolution, X-ray Crystallography and In Silico Docking.

Jérémy Loyau, Gérard Didelot, Pauline Malinge, Ulla Ravn, Giovanni Magistrelli, Jean-François Depoisier, Guillemette Pontini, Yves Poitevin, Marie Kosco-Vilbois, Nicolas Fischer, Stéphane Thore, François Rousseau

Index: J. Mol. Biol. 427 , 2647-62, (2015)

Full Text: HTML

Abstract

Hu 15C1 is a potent anti-human Toll-like receptor 4 (TLR4) neutralizing antibody. To better understand the molecular basis of its biological activity, we used a multidisciplinary approach to generate an accurate model of the Hu 15C1-TLR4 complex. By combining site-directed mutagenesis, in vitro antibody evolution, affinity measurements and X-ray crystallography of Fab fragments, we identified key interactions across the Hu 15C1-TLR4 interface. These contact points were used as restraints to predict the structure of the Fab region of Hu 15C1 bound to TLR4 using computational molecular docking. This model was further evaluated and validated by additional site-directed mutagenesis studies. The predicted structure of the Hu 15C1-TLR4 complex indicates that the antibody antagonizes the receptor dimerization necessary for its activation. This study exemplifies how iterative cycles of antibody engineering can facilitate the discovery of components of antibody-target interactions. Copyright © 2015 Elsevier Ltd. All rights reserved.