Plant and Cell Physiology 2006-04-01

Change in the actin cytoskeleton during seismonastic movement of Mimosa pudica.

Nobuyuki Kanzawa, Yoshinori Hoshino, Makiko Chiba, Daisuke Hoshino, Hidetaka Kobayashi, Naomi Kamasawa, Yoshiro Kishi, Masako Osumi, Masazumi Sameshima, Takahide Tsuchiya

Index: Plant Cell Physiol. 47(4) , 531-9, (2006)

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Abstract

The seismonastic movement of Mimosa pudica is triggered by a sudden loss of turgor pressure. In the present study, we compared the cell cytoskeleton by immunofluorescence analysis before and after movement, and the effects of actin- and microtubule-targeted drugs were examined by injecting them into the cut pulvinus. We found that fragmentation of actin filaments and microtubules occurs during bending, although the actin cytoskeleton, but not the microtubules, was involved in regulation of the movement. Transmission electron microscopy revealed that actin cables became loose after the bending. We injected phosphatase inhibitors into the severed pulvinus to examine the effects of such inhibitors on the actin cytoskeleton. We found that changes in actin isoforms, fragmentation of actin filaments and the bending movement were all inhibited after injection of a tyrosine phosphatase inhibitor. We thus propose that the phosphorylation status of actin at tyrosine residues affects the dynamic reorganization of actin filaments and causes seismonastic movement.