Methods in Molecular Biology 2013-01-01

Determination of monosaccharides derivatized with 2-aminobenzoic Acid by capillary electrophoresis.

Mitsuru Abo, Li-Ping He, Kae Sato, Akira Okubo

Index: Methods Mol. Biol. 984 , 45-50, (2013)

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Abstract

Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate-50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.