European Journal of Pharmaceutical Sciences 2014-12-18

Polymer complex of WR 2721. Synthesis and radioprotective efficiency.

Neli Koseva, Ivelina Tsacheva, Violeta Mitova, Elitsa Vodenicharova, Jessica Molkentine, Kathy Mason, Kolio Troev

Index: Eur. J. Pharm. Sci. 65 , 9-14, (2014)

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Abstract

Polymer complex constructed from WR 2721 and poly(hydroxyoxyethylene phosphate) was synthesized. The structure of complex formed was elucidated by (1)H-, (13)C, (31)P NMR and FT-IR spectroscopy. The radioprotector was immobilized via ionic bonds. Radioprotective efficacy was evaluated by clonal survival of stem cells in crypts of mouse small intestine, and incidence and latency of the acute radiation induced bone marrow syndrome. Protection factors were assessed for WR 2721 and for the polymer complex. Protection factors for the polymer complex ranged from 2.6 for intestinal stem cell survival to 1.35 for 30 day survival (LD50) following whole body radiation exposure. In all cases, the polymer complex was a significantly better radiation protector than the parent compound.Copyright © 2014 Elsevier B.V. All rights reserved.