Artificial Cells, Blood Substitutes and Biotechnology 2012-12-01

Synthesis of microwave-assisted poly(methyl vinyl ether-co-maleic anhydride)-bovine serum albumin bioconjugates.

Mesut Karahan, Sevecen Tuğlu, Zeynep Mustafaeva

Index: Artif. Cells Blood Substit. Immobil. Biotechnol. 40(6) , 363-8, (2012)

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Abstract

The water-soluble poly(methyl vinyl ether-co-maleic anhydride) copolymer-bovine serum albumin bioconjugates were synthesized in the presence of 1-ethyl-3-(3-dimetilamino-propyl) carbodiimide hydrochloride as cross-linking agents via microwave-assisted and conventional methods and characterized by size-exclusion chromatography and high-performance liquid chromatography. According to size-exclusion chromatography and high-performance liquid chromatography results, the bioconjugates synthesized in the microwave-assisted method are more stable and efficient than the conventional method. The reaction time is shortened from 17 hours to 15 minutes by means of the microwave-assisted method.