Analytical Biochemistry 1986-10-01

A sensitive bioluminescence assay for myo-inositol.

T W Gudermann, T G Cooper

Index: Anal. Biochem. 158(1) , 59-63, (1986)

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Abstract

A specific, sensitive bioluminescence assay for myo-inositol is described employing myo-inositol dehydrogenase linked to a commercial NADH bioluminescence kit. Optimum conditions providing a linear response over 4 orders of magnitude are presented with reproducibility of 6% CV and a sensitivity of 1 pmol. The assay is suitable for the measurement of inositol at low concentrations or in small volumes.