Native FKBP12 engineering by ligand-directed tosyl chemistry: labeling properties and application to photo-cross-linking of protein complexes in vitro and in living …

T Tamura, S Tsukiji, I Hamachi

Index: Tamura, Tomonori; Tsukiji, Shinya; Hamachi, Itaru Journal of the American Chemical Society, 2012 , vol. 134, # 4 p. 2216 - 2226

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Citation Number: 43

Abstract

The ability to modify target “native”(endogenous) proteins selectively in living cells with synthetic molecules should provide powerful tools for chemical biology. To this end, we recently developed a novel protein labeling technique termed ligand-directed tosyl (LDT) chemistry. This method uses labeling reagents in which a protein ligand and a synthetic probe are connected by a tosylate ester group. We previously demonstrated its ...