Accelerated photobleaching of a cyanine dye in the presence of a ternary target DNA, PNA probe, dye catalytic complex: a molecular diagnostic

…, R Holmes-Davis, Z Rafinski, B Jedrzejewska…

Index: Wang; Holmes-Davis; Rafinski; Jedrzejewska; Choi; Zwick; Bupp; Izmailov; Paczkowski; Warner; Koshinsky Analytical Chemistry, 2009 , vol. 81, # 6 p. 2043 - 2052

Full Text: HTML

Citation Number: 21

Abstract

In many settings, molecular testing is needed but unavailable due to complexity and cost. Simple, rapid, and specific DNA detection technologies would provide important alternatives to existing detection methods. Here we report a novel, rapid nucleic acid detection method based on the accelerated photobleaching of the light-sensitive cyanine dye, 3, 3′- diethylthiacarbocyanine iodide (DiSC2 (3) I−), in the presence of a target genomic DNA ...