Abstract A crude cuticular extract from both sexes of 3660 fruit flies (Drosophila pallidosa) was subjected to SiO 2 and AgNO 3/SiO 2 column chromatography, accompanied by bioassay for the sex pheromone activity. After three chromatographic steps, the active fraction was obtained. The main component of the active fraction was determined to be (Z, Z)- 5, 27-tritriacontadiene [(Z, Z)-5, 27-C 33: 2, on the basis of gas-liquid chromatographic ...
