Abstract 2-Amino-4-ethoxycarbonylpyridine 1 was used as a starting material in the synthesis of some 4-substituted-N 1-2-pyridylsulfanilamide derivatives to evaluate their antimicrobial activity. The obtained compounds were of no particular effect against the tested organisms except for a noticeable inhibition of B. subtilis, which was of varying extents but remained clearly significant.