Protease-catalyzed peptide formation under high pressure

S Kunugi, K Tanabe, K Yamashita, Y Morikawa…

Index: Kunugi, Shigeru; Tanabe, Kazuo; Yamashita, Kouji; Morikawa, Yoshio; Ito, Takanobu; et al. Bulletin of the Chemical Society of Japan, 1989 , vol. 62, # 2 p. 514 - 518

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Citation Number: 31

Abstract

The effect of high pressure on peptide formation by the catalysis of carboxypeptidase Y (substitution of ester or peptide by amino acid derivative) or by thermolysin (condensation of N-acylamino acid and amino acid amide) was studied. The carboxypeptidase Y-catalyzed substitution reaction of N-[3-(2-furyl) acryloyl] phenylalanine ethyl ester with glycinamide or phenylalaninamide showed a six-fold higher total peptide yield at 200 MPa than at ...