A novel two-photon fluorescence probe for Zn2+ derived from dicyanostilbene as a two- photon fluorophore and 4-(pyridine-2-ylmethyl) piperazine as a novel Zn2+ ligand was developed. The probe shows a 72.5-fold fluorescence enhancement in response to Zn2+, a large two-photon action cross-section (580 GM), a noncytotoxic effect, and pH insensitivity in the biologically relevant range, and its dissociation constant (K dTP) is 0.52±0.01 μM. The ...
