The naturally occurring ecdysteroids 2-deoxy-20-hydroxyecdysone (2-D-20-ECD, 2- deoxycrustecdysone), 2-D-20-ECD 3-acetate (2-deoxyecdysterone 3-acetate), 2-D-20-ECD 22-acetate and 2-D-20-ECD 22-benzoate have been partially synthesized from 20- hydroxyecdysone. Moulting hormone activity of these ecdysteroids has been studied using the Musca bioassay.