Tetrahedron

… of strategies for the site-specific in vivo incorporation of photoreactive amino acids: p-azidophenylalanine, p-acetylphenylalanine and benzofuranylalanine

…, X Doisy, KH Kim, M Praetorius-Ibba, PE Nielsen…

Index: Behrens, Carsten; Nielsen, Jens N.; Fan, Xue-Jun; Doisy, Xavier; Kim, Ki-Hyun; Praetorius-Ibba, Mette; Nielsen, Peter E.; Ibba, Michael Tetrahedron, 2000 , vol. 56, # 48 p. 9443 - 9449

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Citation Number: 28

Abstract

A major limitation of conventional site-directed mutagenesis is that substitutions are restricted to the 20 naturally occurring amino acids. While this problem can be circumvented in vitro to allow the site-specific incorporation of non-canonical amino acids, no similar in vivo methodologies yet exist. The main requirement for such a system is an aminoacyl-tRNA synthetase able to exclusively recognize a non-canonical amino acid and a suppressor ...