i-OHAP, a major metabolite of aprindine (AP), was isolated by TLC from rat feces and identified as N-[3-(N, N-diethylamino) propyl]-N-phenyl-2-aminoindan-5-ol, based on 1 H- NMR, the HH correlation spectroscopy (COSY) spectrum, MS and LC-MS. Its structure was also confirmed by comparison with the synthesized compound. The hydroxy group of i- OHAP was located at the 5-position of the indan ring. AP is a prochiral compound, and the ...