An aldolase antibody, 24H6, which was obtained from immunization with the large hapten 2, is shown to possess an active-site lysine residue with a perturbed p K a of 7.0. This antibody catalyzes both the aldol addition and the retrograde aldol fragmentation with a broad range of substrates that are structurally different from the hapten. This observation suggests that in reactive immunization with 1, 3-diketones, the hapten structure governs the chemistry but ...
[Johnson, Timothy A.; Jang, Doo Ok; Slafer, Brian W.; Curtis, Michael D.; Beak, Peter Journal of the American Chemical Society, 2002 , vol. 124, # 39 p. 11689 - 11698]
