Phosphorylation of ribavirin and viramidine by adenosine kinase and cytosolic 5′-nucleotidase II: implications for ribavirin metabolism in erythrocytes

…, G Larson, H Walker, JH Shim, Z Hong

Index: Wu, Jim Zhen; Larson, Gary; Walker, Heli; Shim, Jae Hoon; Hong, Zhi Antimicrobial Agents and Chemotherapy, 2005 , vol. 49, # 6 p. 2164 - 2171

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Citation Number: 44

Abstract

ABSTRACT Many nucleoside analog drugs, such as ribavirin and viramidine, are activated or metabolized in vivo through 5′-phosphorylation. In this report, we determined the steady- state kinetic parameters for 5′-monophosphorylation of ribavirin and viramidine by adenosine kinase. The apparent K m for ribavirin is 540 μM, and k cat is 1.8 min− 1. Its catalytic efficiency of 3.3× 10− 3 min− 1· μM− 1 is 1,200-fold lower than that of adenosine. ...