Abstract The synthesis of one C (4)-spin-labeled uridine-5′-diphosphate (C (4)-UDP) and two nitroxide-containing 2′-deoxyuridine-5′-triphosphate (dUTP) analogs are reported. The C (4)-UDP derivative was incorporated into copolymers by polynucleotide phosphoxylase (PNPase); one of the two dUTP analogs, substituted at position C (4), was a good substrate for TdT, whereas the other one, substituted at position C (5), served as ...