Cooperativity is an important operation mode for regulatory proteins with multiple binding sites.[1–3] The interdependence of stepwise binding events in such multi-subunit receptors is manifested by a sigmoidal binding isotherm, in which a sharp change in the response curve relates the “on” and “off” states that are separated by a narrow concentration window (Figure 1 a). Extreme cooperativity in homotropic allosterism results in a binary-function- ...
