Process Biochemistry

Enantioselective synthesis of (S)-phenylephrine by whole cells of recombinant Escherichia coli expressing the amino alcohol dehydrogenase gene from Rhodococcus …

WD Lin, CY Chen, HC Chen, WH Hsu

Index: Lin, Wei-De; Chen, Chien-Yu; Chen, Huei-Chung; Hsu, Wen-Hwei Process Biochemistry, 2010 , vol. 45, # 9 p. 1529 - 1536

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Citation Number: 12

Abstract

(R)-phenylephrine [(R)-PE] is an α1-adrenergic receptor agonist that is widely used in over- the-counter drugs to treat the common cold. We found that Rhodococcus erythropolis BCRC 10909 can convert detectable level of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone (HPMAE) to (S)-PE by high performance liquid chromatography tandem mass spectrometry analysis. An amino alcohol dehydrogenase gene (RE_AADH) which possesses the ability ...