Synthesis of Leishmania cap-4 intermediates, cap-2 and cap-3

…, M Shapira, R Stolarski, E Darzynkiewicz

Index: Lewdorowicz, Magdalena; Stepinski, Janusz; Kierzek, Ryszard; Jemielity, Jacek; Zuberek, Joanna; Yoffe, Yael; Shapira, Michal; Stolarski, Ryszard; Darzynkiewicz, Edward Nucleosides, Nucleotides and Nucleic Acids, 2007 , vol. 26, # 10-12 p. 1339 - 1348

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Abstract

Synthesis of Leishmania mRNA 5′-cap analogs, m 7 Gpppm 2 6 AmpAm (cap-2), and m 7 Gpppm 2 6 AmpAmpCm (cap-3) is reported. Binding affinities of those cap analogs for LeishIF4E proteins were determined using fluorescence spectroscopy. Cap-3 showed similar affinity to LeishIF4Es compared to the mature trypanosomatids cap structure (cap-4).