Bioorganic & medicinal chemistry letters

Effects of 8-chlorodeoxyadenosine on DNA synthesis by the klenow fragment of DNA polymerase I

LS Chen, MH Bahr, TL Sheppard

Index: Chen, Lisa S.; Bahr, Michael H.; Sheppard, Terry L. Bioorganic and Medicinal Chemistry Letters, 2003 , vol. 13, # 9 p. 1509 - 1512

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Citation Number: 7

Abstract

8-Chloro-2′-deoxyadenosine (8-Cl-dAdo) was incorporated into synthetic DNA oligonucleotides to determine its effects on DNA synthesis by the 3′–5′ exonuclease-free Klenow fragment of Escherichia coli DNA Polymerase I (KF–). Single nucleotide insertion experiments were used to determine the coding potential of 8-Cl-dAdo in a DNA template. KF–inserted TTP opposite 8-Cl-dAdo in the template, but with decreased efficiency ...