Generation of broad specificity antibodies for sulfonamide antibiotics and development of an enzyme-linked immunosorbent assay (ELISA) for the analysis of milk …

…, H Font, JM Diserens, F Sánchez-Baeza…

Index: Adrian, Javier; Font, Hector; Diserens; Sanchez-Baeza, Francisco; Marco, M.-Pilar Journal of Agricultural and Food Chemistry, 2009 , vol. 57, # 2 p. 385 - 394

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Citation Number: 63

Abstract

Immunoreagents appropriately produced to detect a wide range of sulfonamide antibiotic congeners have been used to develop a highly sensitive enzyme-linked immunosorbent assay (ELISA). The selectivity has been achieved by combining antibodies raised against 5- [6-(4-aminobenzenesulfonylamino) pyridin-3-yl]-2-methylpentanoic acid (SA1), covalently coupled to horseshoe crab hemocyanin (HCH), and 5-[4-(amino) phenylsulfonamide]-5- ...