... Volume 6, Issue 4, 2016, 1356-1379 ... Incubator for cell culture (MCO-15AC, Sanyo), centrifuge (CN 2060, Hsiangtai Co) and microplate photometer (Multiskan FC, Thermo Scientific) were used for carrying out the ... The reaction mixture was refluxed for 40 h at 100-110oC. ...