Characterization of a transcriptional regulatory gene involved in dibenzofuran degradation by Nocardioides sp. strain DF412

P Sukda, N Gouda, E Ito, K Miyauchi…

Index: Sukda, Parichat; Gouda, Nao; Ito, Emi; Miyauchi, Keisuke; Masai, Eiji; Fukuda, Masao Bioscience, Biotechnology and Biochemistry, 2009 , vol. 73, # 3 p. 508 - 516

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Citation Number: 7

Abstract

Nocardioides sp. DF412 degrades dibenzofuran (DF) to salicylate through the sequential actions of DF dioxygenase (dfdA), extradiol dioxygenase (dfdB), and hydrolase (dfdC). The involvement of a TetR-type regulator gene dfdS in the dfdB and dfdS gene expression was investigated. A reporter assay using a luciferase gene indicated repression of dfdB and dfdS promoter activities in the presence of the dfdS gene product, DfdS. Gel shift analysis ...