Lathyrus cicera copper amine oxidase (LCAO) rapidly formed the typical Cu (I)-TPQ semiquinone UV–visible spectrum, identical to that formed by other substrates, upon O2 exhaustion by turnover with excess tryptamine. A new band at 630nm formed more slowly, with intensity dependent on aldehyde and H2O2 concentrations. On prolonged incubation, all bands decayed in parallel, together with loss of enzymatic activity. The blue color ...
