Biochemistry

Mechanistic studies of Bacillus subtilis QueF, the nitrile oxidoreductase involved in queuosine biosynthesis

BWK Lee, SG Van Lanen, D Iwata-Reuyl

Index: Migawa, Michael T.; Hinkley, Jack M.; Hoops, Geoffrey C.; Townsend, Leroy B. Synthetic Communications, 1996 , vol. 26, # 17 p. 3317 - 3322

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Citation Number: 43

Abstract

... Bacillus subtilis orthologue) that is also conserved in GTP cyclohydrolase I (FolE), another T-fold member ... NADPH, 0.025 μM QueF, and variable preQ 0 (0.066−2.0 μM) at 30 °C. The reaction ... specified, aliquots of 50 μL were transferred to an equivalent volume of 0.3 M HCl and ...