... were added to the enzyme mixture of glucuronidase and sulfatase and incubated at 37 °C for 3 h. After ... JEOL LC-12 ESR cuvette, internal size of 60 mm × 10 mm × 0.31 mm, effective volume of 160 ... 15 μL of DMPO, and 50 μL of xanthine oxidase (0.4 unit/mL) were put into a test ...