e.g. Filippa Pettersson or Cancer Res. 75(6) , 1102-12, (2015) or 10.1002/anie.201600521
The Determination of the Concentration of Hydrolytic Enzyme Solutions: α-Chymotrypsin, Trypsin, Papain, Elastase, Subtilisin, and Acetylcholinesterase1
…, FJ Kézdy, JV Killheffer Jr, TH Marshall…
Index: Bender; Begue-Canton; Blakeley; Brubacher; Feder; Gunter; Kezdy; Killheffer Jr.; Marshall; Miller; Roeske; Stoops Journal of the American Chemical Society, 1966 , vol. 88, # 24 p. 5890 - 5913
Abstract: The use of rate assays is contrasted with the use of titrants to determine the absolute concentration of active sites of hydrolytic enzymes. Arguments are given which lead to the preference of a stoichiometric titration for the determination of the concentration of enzyme active sites. The absolute standard in this method is the small organic titrant rather than the enzyme which must be the absolute standard in a rate assay procedure. ...
