Novel substrate specificity of designer 3-isopropylmalate dehydrogenase derived from Thermus thermophilus HB8

…, H Tamegai, T Eguchi, K Kakinuma

Index: Fujita, Masaaki; Tamegai, Hideyuki; Eguchi, Tadashi; Kakinuma, Katsumi Bioscience, Biotechnology and Biochemistry, 2001 , vol. 65, # 12 p. 2695 - 2700

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Citation Number: 4

Abstract

Redesigning of an enzyme for a new catalytic reaction and modified substrate specificity was exploited with 3-isopropylmalate dehydrogenase (IPMDH). Point-mutation on Gly-89, which is not in the catalytic site but near it, was done by changing it to Ala, Ser, Val, and Pro, and all the mutations changed the substrate specificity. The mutant enzymes showed higher catalytic efficiency (kcat/Km) than the native IPMDH when malate was used as a substrate ...