Tetrahedron

Synthesis of methylketone containing nucleoside triphosphates for RNA labelling

E Trévisiol, E Defrancq, J Lhomme, A Laayoun, P Cros

Index: Trevisiol; Defrancq; Lhomme; Laayoun; Cros Tetrahedron, 2000 , vol. 56, # 35 p. 6501 - 6510

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Citation Number: 27

Abstract

In a recent paper, 4 we proposed an alternative procedure for labelling RNA targets, called 'post-amplification labelling'. The approach first involves enzymatic incorporation of a nucleotide triphosphate previously modified so as to carry a reactive functional group X. The resulting amplified RNA sequences are then labelled by reaction with a fluorophore functionalised by a Y group complementary to X. Severe limitations exist as to the nature of X and Y. The conjugation ...