Organic & biomolecular chemistry

Targeting DNA with “light-up” pyrimidine triple-helical forming oligonucleotides conjugated to stabilizing fluorophores (LU-TFOs)

BL Renard, R Lartia, U Asseline

Index: Renard, Brice-Loic; Lartia, Remy; Asseline, Ulysse Organic and Biomolecular Chemistry, 2008 , vol. 6, # 23 p. 4413 - 4425

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Citation Number: 12

Abstract

The synthesis of triple-helix-forming oligonucleotides (TFOs) linked to a series of cyanine monomethines has been performed. Eight cyanines including one thiocyanine, four thiazole orange analogues, and three quinocyanines were attached to the 5′-end of 10-mer pyrimidine TFOs. The binding properties of these modified TFOs with their double-stranded DNA target were studied by absorption and steady-state fluorescence spectroscopy. The ...