Carbohydrate research

Synthesis of acceptor substrate analogs for the study of glycosyltransferases involved in the second step of the biosynthesis of O-antigen repeating units

JG Riley, C Xu, I Brockhausen

Index: Riley, John G.; Xu, Changchang; Brockhausen, Inka Carbohydrate Research, 2010 , vol. 345, # 5 p. 586 - 597

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Citation Number: 11

Abstract

O-antigens of Gram negative bacteria are polysaccharides covalently attached to lipopolysaccharides (LPS) that have roles as virulence factors. Due to the lack of defined substrates for in vitro assays only a few of the enzymes involved in the biosynthesis of O- antigens have been studied. Many O-antigens have GlcNAc at the reducing end of the oligosaccharide chain linked to pyrophosphate-lipid. We therefore designed and ...