An LC/MS/MS method for stable isotope dilution studies of β-carotene bioavailability, bioconversion, and vitamin A status in humans

…, P Berry, GA Taylor, J Cowell, MJ Hall, J Hesketh…

Index: Oxley, Anthony; Berry, Philip; Taylor, Gordon A.; Cowell, Joseph; Hall, Michael J.; Hesketh, John; Lietz, Georg; Boddy, Alan V. Journal of Lipid Research, 2014 , vol. 55, # 2 p. 319 - 328

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Citation Number: 16

Abstract

Abstract Isotope dilution is currently the most accurate technique in humans to determine vitamin A status and bioavailability/bioconversion of provitamin A carotenoids such as β- carotene. However, limits of MS detection, coupled with extensive isolation procedures, have hindered investigations of physiologically-relevant doses of stable isotopes in large intervention trials. Here, a sensitive liquid chromatography-tandem mass spectrometry (LC ...