Carbohydrate research

UDP-6-deoxy-6-fluoro-α-d-galactose binds to two different galactosyltransferases, but neither can effectively catalyze transfer of the modified galactose to the …

CL Schengrund, P Kováč

Index: Schengrund, Cara-Lynne; Kovac, Pavol Carbohydrate Research, 1999 , vol. 319, # 1-4 p. 24 - 28

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Citation Number: 22

Abstract

The effect of substitution of the HO-6 of d-galactose with fluorine on the ability of α-(1→ 3)- galactosyltransferase (EC 2.4. 1.151) and β-(1→ 4)-galactosyltransferase (EC 2.4. 1.22) to catalyze its transfer from UDP to an appropriate acceptor was determined. HPLC analyses indicated that each transferase properly catalyzed formation of the expected product [β-d- Gal-(1→ 4)-d-GlcNAc] for the β-(1→ 4)-galactosyltransferase and α-d-Gal-(1→ 3)-β-d-Gal- ...