Equilibration of tyrosyl radicals (Y356•, Y731•, Y730•) in the radical propagation pathway of the Escherichia coli class Ia ribonucleotide reductase

…, B Corzilius, RG Griffin, JA Stubbe

Index: Minnihan, Ellen C.; Young, Douglas D.; Schultz, Peter G.; Stubbe, Joanne Journal of the American Chemical Society, 2011 , vol. 133, # 40 p. 15942 - 15945

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Citation Number: 40

Abstract

Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleotides to deoxynucleotides using a diferric tyrosyl radical (Y122•) cofactor in β2 to initiate catalysis in α2. Each turnover requires reversible long-range proton-coupled electron transfer (PCET) over 35 Å between the two subunits by a specific pathway (Y122•⇆[W48?]⇆ Y356 within β to Y731⇆ Y730⇆ C439 within α). Previously, we reported that a β2 mutant ...