Alcaligenes sp. T501, which has the ability to produce l-malate from maleate at a concentration of 20% in a molar yield of 98.4%, was isolated from soil. Analysis of the reaction mixture components suggested that maleate was converted to l-malate via fumarate through the action of maleate isomerase and fumarase. These two enzymes were constitutively found in the cells regardless of the carbon source in the medium. The ...
