In this study, some novel 2, 6-disubstituted imidazo [2, 1-b][1, 3, 4] thiadiazoles 4 (a–i), 7 (a– p) and 11 (a–i) were synthesized from 5-substituted-1, 3, 4-thiadiazol-2-amine. The newly synthesized compounds 4a, 4b, 4c, 4e, 4g, 7j, 7l, 11b and 11c were evaluated in the National Cancer Institute for single dose in vitro primary cytotoxicity assay. Among the tested nine compounds, compound 4b (107166/760239) and 4c (107168/760240) were passed ...