... The final product was lyophilized and used for identification by spectral and chemical analyses. ... niger IX-glucosidase in a total volume of 200 J.LI of 0.1 M acetate buffer (pH 5.3) and incubated at 50°C for 3 hr. ... 21) c. G. Mead and FJ Finamore, Biochemistry, 8, 2652 (1969). ...