Identification and quantitation of the lesion accompanying base release in bleomycin-mediated DNA degradation

LE Rabow, JA Stubbe, JW Kozarich

Index: Rabow; Stubbe; Kozarich Journal of the American Chemical Society, 1990 , vol. 112, # 8 p. 3196 - 3203

Full Text: HTML

Citation Number: 89

Abstract

Abstract: Interaction of bleomycin, Fe2+, and limiting O2 or bleomycin, Fe3+, and H202 with DNA results in the production of free nucleic acid base and an oxidatively damaged sugar lesion that undergoes strand scission subsequent to treatment with alkali. The hexamer d (CGCGCG) has been utilized to develop a protocol to establish the identity of this lesion as a 2'-deoxy-4'-pentulose moiety and to establish its stoichiometric production with respect to ...