The synthesis of 11 new N-substituted derivatives of (E)-4′-hydroxy-3′- methoxystilbazoles-4 and their antimicrobial activity are reported. The relationships between structure and antibacterial activity are studied. In particular, compounds 5, 7 and 8 showed good antibacterial activity against Staphylococcusaureus, Streptococcus faecalis and Bacillus subtilis, while compound 6 did so against S. aureus and S. faecalis.