Click chemistry facilitates formation of reporter ions and simplified synthesis of amine-reactive multiplexed isobaric tags for protein quantification

…, GT Smith, S Hess, G Czerwieniec, JA Loo…

Index: Sohn, Chang Ho; Lee, J. Eugene; Sweredoski, Michael J.; Graham, Robert L.J.; Smith, Geoffrey T.; Hess, Sonja; Czerwieniec, Gregg; Loo, Joseph A.; Deshaies, Raymond J.; Beauchamp Journal of the American Chemical Society, 2012 , vol. 134, # 5 p. 2672 - 2680

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Citation Number: 25

Abstract

We report the development of novel reagents for cell-level protein quantification, referred to as Caltech isobaric tags (CITs), which offer several advantages in comparison with other isobaric tags (eg, iTRAQ and TMT). Click chemistry, copper (I)-catalyzed azide–alkyne cycloaddition (CuAAC), is applied to generate a gas-phase cleavable linker suitable for the formation of reporter ions. Upon collisional activation, the 1, 2, 3-triazole ring constructed ...