Abstract A series of novel cycloalkylthiophene–imine derivatives containing benzothiazole unit were designed, synthesized and evaluated for their anti-viral activities. The bio- evaluation results indicated that some of the target compounds (such as 5g, 5i, 5u) exhibited good to moderate antiviral effect on CVB5, ADV7 and EV71 viruses, however, these compounds did not have inhibition activity against H1N1 virus. Especially, the compounds ...