Journal of Molecular Catalysis B: Enzymatic

Enzymatic preparation of a natural sweetener rubusoside from specific hydrolysis of stevioside with β-galactosidase from Aspergillus sp.

G Tao, D Kim, Y Xia

Index: Wan, Hui-Da; Tao, Guan-Jun; Kim, Doman; Xia, Yong-Mei Journal of Molecular Catalysis B: Enzymatic, 2012 , vol. 82, p. 12 - 17

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Citation Number: 11

Abstract

Rubusoside is a precious bioactive sweetener which mainly exists in Chinese sweet tea plant (Rubus suavissimus S. Lee), while stevioside is an abundant natural sweetener with bitter aftertaste. In this work, a β-galactosidase from Aspergillus sp. presented specific hydrolytic activity on β-1, 2 glucosidic linkage of stevioside, and converting stevioside to rubusoside. The hydrolytic activity was weak on the natural stevioside analogs, which ...