Development of an immunoassay for the residues of the herbicide bensulfuron-methyl

JK Lee, KC Ahn, OS Park, YK Ko…

Index: Lee, Jae Koo; Ahn, Ki Chang; Park, Oee Sook; Ko, Yong Kwan; Kim, Dae-Whang Journal of agricultural and food chemistry, 2002 , vol. 50, # 7 p. 1791 - 1803

Full Text: HTML

Citation Number: 70

Abstract

To develop a competitive indirect enzyme-linked immunosorbent assay based on polyclonal antibodies for the detection of the sulfonylurea herbicide bensulfuron-methyl, seven structurally related haptens were synthesized. Four of them mimicking the target analyte were conjugated to keyhole limpet hemocyanin by the N-hydroxysuccinimide activated ester method to use as immunogens, and all of them were conjugated to bovine serum albumin ...