Three distinct series of substituted pyrazole blockers of divalent metal transporter 1 (DMT1) were elaborated from the high-throughput screening pyrazolone hit 1. Preliminary hit-to-lead efforts revealed a preference for electron-withdrawing substituents in the 4-amido-5- hydroxypyrazole series 6a–l. In turn, this preference was more pronounced in a series of 4- aryl-5-hydroxypyrazoles 8a–j. The representative analogs 6f and 12f were found to be ...