Abstract Analysis of the methanol extracts of the rhizomes of Iris milesii resulted in the isolation of a new isoflavone, 5, 6, 7, 4′-tetrahydroxy-3′-methoxyisoflavone and that of Iris kumaonensis, iriskumaonin methyl ether, iriskumaonin, irisflorentin, junipegenin-A, irigenin and iridin.