Abstract 1 H-decoupled 19 F NMR has been used to monitor the highly regioselective oxidation of a fluorine-tagged thia-fatty acid derivative by castor stearoyl-ACP Δ 9 desaturase. The major enzymatic product, after reductive work-up, was identified as 9-fluoro- 1-nonanol. This compound could be easily distinguished from substrate and a 9-sulfoxy by- product on the basis of its 19 F NMR chemical shift and spiking experiments using ...
